Rapid transport of the acidic phosphoproteins ofPlasmodium berghei andP. chabaudi from the intraerythrocytic parasite to the host membrane using a miniaturized fractionation procedure

@article{Wiser1992RapidTO,
  title={Rapid transport of the acidic phosphoproteins ofPlasmodium berghei andP. chabaudi from the intraerythrocytic parasite to the host membrane using a miniaturized fractionation procedure},
  author={Mark F. Wiser and H. Norbert Lanners},
  journal={Parasitology Research},
  year={1992},
  volume={78},
  pages={193-200}
}
A miniaturized procedure for the separation of the host erythrocyte membrane from malarial parasites based on saponin lysis and density-gradient centrifugation with Percoll is described. The procedure requires only 20–35 μl packed infected erythrocytes, is simple to perform, needs no sophisticated equipment, and can be completed in <2 h. Analysis of the isolated erythrocyte membranes and parasites using marker enzymes and electron microsropy revealed that both the purity and the yield of these… CONTINUE READING

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Phosphoproteins associated with the host erythrocyte membrane during Plasmodium chabaudi infection

  • MF Wiser
  • 1987
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