Rapid subtyping of dengue viruses by restriction site-specific (RSS)-PCR.

  title={Rapid subtyping of dengue viruses by restriction site-specific (RSS)-PCR.},
  author={Eva Harris and Erick Sandoval and Ana M Xet-Mull and Michael Johnson and Lee W Riley},
  volume={253 1},
Dengue is a major public health problem worldwide. It is caused by four dengue virus serotypes, each further divided into distinct genetic subtypes. Strain typing is important for understanding the epidemiology and viral factors associated with disease transmission. However, most of the existing subtyping methods are expensive and technically unwieldy for timely, practical applications in developing countries. Here we describe a simple, rapid, PCR-based subtyping method, restriction site… CONTINUE READING

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