Rapid spectrophotometric technique for quantifying iron in cells labeled with superparamagnetic iron oxide nanoparticles: potential translation to the clinic.

@article{Dadashzadeh2013RapidST,
  title={Rapid spectrophotometric technique for quantifying iron in cells labeled with superparamagnetic iron oxide nanoparticles: potential translation to the clinic.},
  author={Esmaeel R Dadashzadeh and Matthew Hobson and L Henry Bryant and Dana D Dean and Joseph A. Frank},
  journal={Contrast media & molecular imaging},
  year={2013},
  volume={8 1},
  pages={50-6}
}
Labeling cells with superparamagnetic iron oxide (SPIO) nanoparticles provides the ability to track cells by magnetic resonance imaging. Quantifying intracellular iron concentration in SPIO labeled cells would allow for the comparison of agents and techniques used to magnetically label cells. Here we describe a rapid spectrophotometric technique (ST) to quantify iron content of SPIO-labeled cells, circumventing the previous requirement of an overnight acid digestion. Following lysis with 10… CONTINUE READING
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