Rapid sizing of individual fluorescently stained DNA fragments by flow cytometry.

  title={Rapid sizing of individual fluorescently stained DNA fragments by flow cytometry.},
  author={P. Goodwin and M. E. Johnson and J. Martin and W. P. Ambrose and B. Marrone and J. H. Jett and R. Keller},
  journal={Nucleic acids research},
  volume={21 4},
  • P. Goodwin, M. E. Johnson, +4 authors R. Keller
  • Published 1993
  • Biology, Medicine
  • Nucleic acids research
  • Large, fluorescently stained restriction fragments of lambda phage DNA are sized by passing individual fragments through a focused continuous wave laser beam in an ultrasensitive flow cytometer at a rate of 60 fragments per second. The size of the fluorescence burst emitted by each stained DNA fragment, as it passes through the laser beam, is measured in one millisecond. One hundred sixty four seconds of fluorescence burst data allow linear sizing of DNA with an accuracy of better than two… CONTINUE READING

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