Rapid separation of dehydrogenases by affinity chromatography with new induced specificity phases.

Abstract

We describe a procedure using immobilized nicotinamide as an affinity chromatographic ligand for the binding of NAD(P)+-dependent dehydrogenases. The procedure involves preparation of nicotinamide N1-(N-(6-aminohexyl)-acetamide)-agarose and modification of the immobilized nicotinamide by the addition of a ketone or an aldehyde to form an adduct. The… (More)

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Cite this paper

@article{Torreilles1986RapidSO, title={Rapid separation of dehydrogenases by affinity chromatography with new induced specificity phases.}, author={Jean Torreilles and Maryse Gu{\'e}rin and Bernard Descomps}, journal={Biochimie}, year={1986}, volume={68 4}, pages={565-8} }