Rapid separation of DNA restriction fragments using capillary electrophoresis.

  title={Rapid separation of DNA restriction fragments using capillary electrophoresis.},
  author={Arieh Sierra Cohen and D R Najarian and Jarrod A Smith and Barry L. Karger},
  journal={Journal of chromatography},
Open-tube capillary electrophoresis has been applied to the separation of restriction fragments of DNA with a Tris-borate buffer containing 7 M urea and 0.1% sodium dodecyl sulfate. The importance of sample pretreatment and of the injection of heated samples has been demonstrated. In one separation, a DNA restriction fragment mixture from 72 to 23,130 base pairs (DRIgestTM III) (molecular weight range from 4.6.10(4) to 1.5.10(7] has been electrophoresed in 10 min on a column of 15 cm effective… CONTINUE READING
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