Rapid selection of donor myoblast clones for muscular dystrophy therapy using cell surface expression of NCAM.

@article{BellesIsles1993RapidSO,
  title={Rapid selection of donor myoblast clones for muscular dystrophy therapy using cell surface expression of NCAM.},
  author={Marthe Belles-Isles and Rabindra K. Roy and G Dansereau and Marlyne Goulet and Brigitte Roy and J. P. Bouchard and Jacques Pierre Tremblay},
  journal={European journal of histochemistry : EJH},
  year={1993},
  volume={37 4},
  pages={375-80}
}
This study describes an easy 3 step-procedure to prepare rapidly and at low cost, pure myoblast cell cultures from a normal muscle biopsy. Following collagenase and trypsin treatment of the tissue (step 1), dissociated cells were cloned at a density of 10 cells/ml in MCDB 120 medium (0.2 ml/well). Clones that grew were then tested for NCAM cell surface expression by cytofluorometric analysis (CFA) using Coulter CD56-PE monoclonal antibodies (step 2). Only those clones with more than 98… CONTINUE READING
Highly Cited
This paper has 20 citations. REVIEW CITATIONS

Citations

Publications citing this paper.
Showing 1-10 of 12 extracted citations

Similar Papers

Loading similar papers…