Rapid selection of aminoacyl-tRNAs based on biotinylation of α-NH 2 group of charged amino acids

Abstract

A rapid selection procedure to separate low amounts of aminoacylated tRNAs from large pools of inactive variants is described. The procedure involves a threestep protocol. After initial aminoacylation of a tRNA pool, N-hydroxysuccinimide ester chemistry is applied to biotinylate the α-NH2 group of the amino acid bound to the 3′-end of a tRNA. The biotin tag… (More)

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