Rapid screening technique for class 1 integrons in Enterobacteriaceae and nonfermenting gram-negative bacteria and its use in molecular epidemiology.

@article{Maguire2001RapidST,
  title={Rapid screening technique for class 1 integrons in Enterobacteriaceae and nonfermenting gram-negative bacteria and its use in molecular epidemiology.},
  author={Anita J. Maguire and Derek F. J. Brown and Jim J. Gray and Ulrich Desselberger},
  journal={Antimicrobial agents and chemotherapy},
  year={2001},
  volume={45 4},
  pages={1022-9}
}
A screening technique for integrons in members of the family Enterobacteriaceae and nonfermenting gram-negative bacteria by real-time PCR is reported. A total of 226 isolates of gram-negative bacteria obtained from a variety of clinical specimens were screened for class 1 integrons by real-time PCR performed on a LightCycler instrument. This technique used a primer pair specific for a 300-bp conserved region at the 5' ends of class 1 integrons. The screening assay was evaluated by comparison… CONTINUE READING