Rapid screening of the aglycone specificity of glycosidases: applications to enzymatic synthesis of oligosaccharides.

@article{Blanchard2001RapidSO,
  title={Rapid screening of the aglycone specificity of glycosidases: applications to enzymatic synthesis of oligosaccharides.},
  author={Jan E. Blanchard and Stephen G Withers},
  journal={Chemistry & biology},
  year={2001},
  volume={8 7},
  pages={627-33}
}
BACKGROUND Retaining glycosidases can catalyse glycosidic bond formation through transglycosylation from a donor sugar to an acceptor bound in the aglycone site. The aglycone specificity of a glycosidase is not easily determined, thereby complicating the choice of the most appropriate glycosidase for use as a catalyst for transglycosylation. We have developed a strategy to rapidly screen the aglycone specificity of a glycosidase and thereby determine which enzymes are best suited to catalyse… CONTINUE READING