Rapid sampling for analysis of in vivo kinetics using the BioScope: a system for continuous-pulse experiments.

@article{Visser2002RapidSF,
  title={Rapid sampling for analysis of in vivo kinetics using the BioScope: a system for continuous-pulse experiments.},
  author={Diana Visser and Gertan A van Zuylen and Jan C van Dam and Arthur Oudshoorn and Michael R Eman and Cor Ras and Walter M. van Gulik and Johannes Frank and Gijs W K van Dedem and Joseph J. Heijnen},
  journal={Biotechnology and bioengineering},
  year={2002},
  volume={79 6},
  pages={674-81}
}
In this article we present a novel device, the BioScope, which allows elucidation of in vivo kinetics of microbial metabolism via perturbation experiments. The perturbations are carried out according to the continuous-flow method. The BioScope consists of oxygen permeable silicon tubing, connected to the fermentor, through which the broth flows at constant velocity. The tubing has a special geometry (serpentine channel) to ensure plug flow. After leaving the fermentor, the broth is mixed with a… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 37 extracted citations

Systems Metabolic Engineering

Springer Netherlands • 2012
View 7 Excerpts
Highly Influenced

Prelude to rational scale-up of penicillin production: a scale-down study

Applied Microbiology and Biotechnology • 2013
View 5 Excerpts
Highly Influenced

Impact of thermodynamic principles in systems biology.

Advances in biochemical engineering/biotechnology • 2010
View 5 Excerpts
Highly Influenced

Metabolomics: current state and evolving methodologies and tools

Applied Microbiology and Biotechnology • 2007
View 5 Excerpts
Highly Influenced

Current state and challenges for dynamic metabolic modeling.

Current opinion in microbiology • 2016
View 3 Excerpts

Similar Papers

Loading similar papers…