Rapid recovery- and characterization of transformants following Agrobacterium-mediated T-DNA transfer to sorghum

@article{Kumar2010RapidRA,
  title={Rapid recovery- and characterization of transformants following Agrobacterium-mediated T-DNA transfer to sorghum},
  author={Vinod Kumar and Leanne M Campbell and Keerti S. Rathore},
  journal={Plant Cell, Tissue and Organ Culture (PCTOC)},
  year={2010},
  volume={104},
  pages={137-146}
}
Although Agrobacterium-mediated transformation of sorghum has been reported, the process is rather lengthy and remains difficult, requiring some very stringent conditions to obtain transformants. We have investigated and describe the parameters related to cocultivation, culture, and regeneration that have allowed us to obtain transgenic sorghum plants in as little as 2.5 months. We observed a 2.9-fold increase in transformation efficiency when L-cysteine was included in the medium during the… CONTINUE READING