Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR.

@article{RodrguezLzaro2004RapidQD,
  title={Rapid quantitative detection of Listeria monocytogenes in meat products by real-time PCR.},
  author={David Rodr{\'i}guez-L{\'a}zaro and Anna Jofr{\'e} and Teresa Aymerich and Marta Hugas and Maria Pla},
  journal={Applied and environmental microbiology},
  year={2004},
  volume={70 10},
  pages={6299-301}
}
We describe a quick and simple method for the quantitative detection of Listeria monocytogenes in meat products. This method is based on filtration, Chelex-100-based DNA purification, and real-time PCR. It can detect as few as 100 CFU/g and quantify as few as 1,000 CFU/g, with excellent accuracy compared to that of the plate count method. Therefore, it is a promising alternative for the detection of L. monocytogenes in meat products.