Rapid quantification of total viral DNA in the supernatants of activated sludge samples with the fluorescent dye PicoGreen.

@article{Otawa2008RapidQO,
  title={Rapid quantification of total viral DNA in the supernatants of activated sludge samples with the fluorescent dye PicoGreen.},
  author={Kenichi Otawa and Hiroyasu Satoh and Yuko Kanai and Motoharu Onuki and Takashi Mino},
  journal={Letters in applied microbiology},
  year={2008},
  volume={46 4},
  pages={434-8}
}
AIMS To develop a rapid and simple method for quantifying viral DNA concentrations and determining viral quantities in activated sludge. METHODS AND RESULTS Activated sludge samples were obtained from three full-scale and one laboratory-scale process. They were centrifuged and the supernatant was filtered through a 0.2-microm membrane filter. Free DNA was removed by DNase-I treatment; any DNA within the viral capsid was liberated by heat treatment and proteinase K, and viral DNA… CONTINUE READING