Rapid quantification of global DNA methylation by isocratic cation exchange high-performance liquid chromatography.

Abstract

The DNA of many eukaryotes is methylated at specific cytosine residues in connection with gene regulation. Here we report a method for the quantification of global cytosine methylation based on enzymatic hydrolysis of DNA, dephosphorylation, and subsequent high-performance cation exchange chromatography. Nucleosides are separated in less than 3 min under… (More)
DOI: 10.1016/j.ab.2008.01.001

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