Rapid purification of intact chloroplasts and heat-bleached ribosome-deficient plastids from rye leaves on discontinuous PercollTM gradients

@article{Hinghaus2005RapidPO,
  title={Rapid purification of intact chloroplasts and heat-bleached ribosome-deficient plastids from rye leaves on discontinuous PercollTM gradients},
  author={R. H{\"o}inghaus and J{\"u}rgen Feierabend},
  journal={Protoplasma},
  year={2005},
  volume={118},
  pages={114-120}
}
The distribution of the major cellular organelles from normal green and heat-bleached 70S ribosome-deficient rye leaves was analyzed by the assay of marker enzymes after isopycnic centrifugation on linear Percoll gradients. A rapid procedure with stepped Percoll gradients was developed for the purification of intact plastids, virtually free of contaminations by mitochondria and peroxisomes. The intact plastids are separated from broken plastids by a density step and from mitochondria and… CONTINUE READING

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