Rapid purification of human peripheral blood monocytes by centrifugation through Ficoll-Hypaque and Sepracell-MN.

@article{Vissers1988RapidPO,
  title={Rapid purification of human peripheral blood monocytes by centrifugation through Ficoll-Hypaque and Sepracell-MN.},
  author={Margret C. M. Vissers and S A Jester and Joseph C. Fantone},
  journal={Journal of immunological methods},
  year={1988},
  volume={110 2},
  pages={203-7}
}
We have developed a rapid and simple method for isolating human peripheral blood monocytes in suspension. The procedure combines two separation media and involves isolation of the mononuclear cells by centrifugation through Ficoll-Hypaque followed by purification of the monocytes using Sepracell-MN, a colloidal silica-based medium. The final cell population contained approximately 90% monocytes with good functional ability. The contaminating cells were lymphocytes. Viability was always greater… CONTINUE READING

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