Rapid purification of cationic granule proteases: application to human granzymes.

@article{Hanna1993RapidPO,
  title={Rapid purification of cationic granule proteases: application to human granzymes.},
  author={William L. Hanna and Xuewu Zhang and Jane Turbov and Ulrike Winkler and Dorothy Hudig and Christopher J. Froelich},
  journal={Protein expression and purification},
  year={1993},
  volume={4 5},
  pages={398-404}
}
This report describes a simple scheme for the simultaneous purification of cationic human granzymes A, B, and 3 from human interleukin 2 (IL-2)-activated lymphocytes. The process, which requires approximately 8 h, includes: (1) cell cavitation, (2) two centrifugation steps, (3) four granule solubilization steps, and (4) cation-exchange chromatography. Granule solubilization consists of three extractions with a hypotonic buffer (25 mM NaCl) that contained Triton X-100 followed by a final… CONTINUE READING

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