Rapid purification and characterization of human platelet glycoprotein V: the amino acid sequence contains leucine-rich repetitive modules as in glycoprotein Ib.

@article{Shimomura1990RapidPA,
  title={Rapid purification and characterization of human platelet glycoprotein V: the amino acid sequence contains leucine-rich repetitive modules as in glycoprotein Ib.},
  author={Taizo Shimomura and Ken-ichi Fujimura and S Maehama and Masumi Takemoto and Kanae Oda and Toshio Fujimoto and Rieko Oyama and Masatatsu Suzuki and Keiko Ichihara-Tanaka and Koiti Titani},
  journal={Blood},
  year={1990},
  volume={75 12},
  pages={2349-56}
}
Glycoprotein V (GPV) is a membrane-associated, 82 Kd platelet glycoprotein that is hydrolyzed during thrombin activation to yield 69 Kd fragment. We have developed a rapid and simple method for isolation of the protein from platelet extracts using a combination of gel permeation, anion-exchange, and lectin affinity chromatography. The partial amino acid… CONTINUE READING