Rapid production of clinical-grade gammaretroviral vectors in expanded surface roller bottles using a "modified" step-filtration process for clearance of packaging cells.

@article{Feldman2011RapidPO,
  title={Rapid production of clinical-grade gammaretroviral vectors in expanded surface roller bottles using a "modified" step-filtration process for clearance of packaging cells.},
  author={Steven Alexander Feldman and Stephanie L. Goff and Hui Xu and Mary A. Black and James N. Kochenderfer and Laura Johnson and James C Yang and Qiong Wang and Maria R. Parkhurst and Scott Cross and Richard Alan Morgan and Kenneth G Cornetta and Steven A Rosenberg},
  journal={Human gene therapy},
  year={2011},
  volume={22 1},
  pages={
          107-15
        }
}
Production of clinical-grade gammaretroviral vectors for ex vivo gene delivery requires a scalable process that can rapidly generate large amounts of vector supernatant, clear large numbers of residual packaging cells with minimal decreases in vector titer, and satisfy all current regulatory guidelines regarding product biosafety. To that end, we have developed a simplified method that is compliant with current good manufacturing practices for the production of clinical-grade gammaretroviral… CONTINUE READING
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