Rapid preimplantation detection of mutant (shiverer) and normal alleles of the mouse myelin basic protein gene allowing selective implantation and birth of live young.

@article{Gomez1990RapidPD,
  title={Rapid preimplantation detection of mutant (shiverer) and normal alleles of the mouse myelin basic protein gene allowing selective implantation and birth of live young.},
  author={Christina M. Gomez and Audrey Muggleton-Harris and David G Whittingham and Leroy E. Hood and Clint Readhead},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1990},
  volume={87 12},
  pages={
          4481-4
        }
}
As a model for the detection of human genetic disease in preimplantation embryos, we describe a method in which trophectoderm biopsy samples from viable mouse blastocysts are simultaneously analyzed for the presence of a normal or mutant allele of the myelin basic protein gene by the polymerase chain reaction. The biopsied embryos are kept in culture during analysis of biopsied material and later reintroduced to a foster mother. Prenatal diagnosis can be completed in less than 7 hr. The… CONTINUE READING

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