Rapid multiplexed flow cytometric assay for botulinum neurotoxin detection using an automated fluidic microbead-trapping flow cell for enhanced sensitivity.

@article{Ozanich2009RapidMF,
  title={Rapid multiplexed flow cytometric assay for botulinum neurotoxin detection using an automated fluidic microbead-trapping flow cell for enhanced sensitivity.},
  author={Richard M. Ozanich and Cynthia J. Bruckner-Lea and Marvin G Warner and Keith Miller and Kathryn C. Antolick and James D Marks and Jianlong Lou and Jay W Grate},
  journal={Analytical chemistry},
  year={2009},
  volume={81 14},
  pages={
          5783-93
        }
}
A bead-based sandwich immunoassay for botulinum neurotoxin serotype A (BoNT/A) has been developed and demonstrated using a recombinant 50 kDa fragment (BoNT/A-HC-fragment) of the BoNT/A heavy chain (BoNT/A-HC) as a structurally valid simulant. Three different anti-BoNT/A antibodies were attached to three different fluorescent dye encoded flow cytometry beads for multiplexing. The assay was conducted in two formats: a manual microcentrifuge tube format and an automated fluidic system format… CONTINUE READING