Rapid multiple shoot production from cotyledonary node explants of pea (Pisum sativum L.)

Abstract

Multiple shoots were produced, from cotyledonary node explants of pea (Pisum sativum L.) cultured on MS medium containing 1 mg 1−1 BAP. The number of buds formed could be increased by scraping the nodes before culture or by increasing the cytokinin concentation. However, cytokinin levels over 5 mg 1−1 increasingly produced shoots that were vitrified and… (More)
DOI: 10.1007/BF02623626

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