Rapid movement of water and cryoprotectants in pig expanded blastocysts via channel processes: its relevance to their higher tolerance to cryopreservation.

@article{Jin2013RapidMO,
  title={Rapid movement of water and cryoprotectants in pig expanded blastocysts via channel processes: its relevance to their higher tolerance to cryopreservation.},
  author={Bo Jin and Ryu-ichi Higashiyama and Yu-ichi Nakata and Jun-ichi Yonezawa and Shangdan Xu and Masashi Miyake and Sei-ichi Takahashi and Kazuhiro Kikuchi and Ken-ichi Yazawa and Shuhei Mizobuchi and Saori Niimi and Mizuho Kitayama and Chihiro Koshimoto and Kazutsugu Matsukawa and Magosaburo Kasai and Keisuke Edashige},
  journal={Biology of reproduction},
  year={2013},
  volume={89 4},
  pages={87}
}
Pig oocytes and embryos are highly sensitive to cryopreservation; however, tolerance to cryopreservation increases in embryos at the expanded blastocyst stage. This increased tolerance may be attributed to a decrease in cytoplasmic lipid droplets at this stage. We previously showed that an increase in the permeability of the plasma membrane in mouse oocytes to water and cryoprotectants, caused by the artificial expression of aquaporin 3, an aquaglyceroporin, enhanced tolerance to… CONTINUE READING
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