Rapid movement of axonal neurofilaments interrupted by prolonged pauses

  title={Rapid movement of axonal neurofilaments interrupted by prolonged pauses},
  author={Lei Wang and Chung-Liang Ho and Dongming Sun and Ronald K. H. Liem and Anthony Brown},
  journal={Nature Cell Biology},
Axonal cytoskeletal and cytosolic proteins are synthesized in the neuronal cell body and transported along axons by slow axonal transport, but attempts to observe this movement directly in living cells have yielded conflicting results. Here we report the direct observation of the axonal transport of neurofilament protein tagged with green fluorescent protein in cultured nerve cells. Live-cell imaging of naturally occurring gaps in the axonal neurofilament array reveals rapid, intermittent and… 

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