Rapid molecular typing of Tuber melanosporum, T. brumale and T. indicum from tree seedlings and canned truffles.

@article{Douet2004RapidMT,
  title={Rapid molecular typing of Tuber melanosporum, T. brumale and T. indicum from tree seedlings and canned truffles.},
  author={J. P. Douet and Michel Castroviejo and D. Mabru and G{\'e}rard Chevalier and Cl{\'e}mence Dupr{\'e} and F Bergougnoux and J{\'e}r{\^o}me Ricard and Bethan Medina},
  journal={Analytical and bioanalytical chemistry},
  year={2004},
  volume={379 4},
  pages={668-73}
}
We have developed new DNA extraction and purification procedures for investigation of mycorrhized seedlings and canned truffles. Use of these procedures on approximately 100 mg initial material enabled good sample representation. For mycorrhized seedlings, Taq polymerase inhibitors were discarded irrespective of tree species. In routine analysis we systematically used consensus primers ITS1/ITS4 to check the absence of Taq polymerase inhibitors and the presence of fungus DNA. Positive response… CONTINUE READING