Rapid molecular typing of Burkholderia pseudomallei, isolated in an outbreak of melioidosis in Singapore in 2004, based on variable-number tandem repeats.

@article{Liu2006RapidMT,
  title={Rapid molecular typing of Burkholderia pseudomallei, isolated in an outbreak of melioidosis in Singapore in 2004, based on variable-number tandem repeats.},
  author={YiChun Liu and J Loh and Lay Tin Aw and Eric P. H. Yap and May Ann Lee and Eng Eong Ooi},
  journal={Transactions of the Royal Society of Tropical Medicine and Hygiene},
  year={2006},
  volume={100 7},
  pages={687-92}
}
An increase in the number of reported melioidosis cases was observed in the first 4 months of 2004. These cases were associated with a significant increase in case-fatality rate compared with the past 5 years. In order to exclude the possibility of a single source, including the possibility of intentional release of Burkholderia pseudomallei, we applied a multiplex PCR-based multilocus variable-number tandem repeat (VNTR) assay to determine the clonality of the clinical isolates. Our… CONTINUE READING
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