Rapid molecular characterization of Clostridium difficile and assessment of populations of C. difficile in stool specimens.

@article{Wroblewski2009RapidMC,
  title={Rapid molecular characterization of Clostridium difficile and assessment of populations of C. difficile in stool specimens.},
  author={Danielle Wroblewski and George E. Hannett and Dianna J. Bopp and Ghinwa K. Dumyati and Tanya A. Halse and Nellie B. Dumas and Kimberlee Aruda Musser},
  journal={Journal of clinical microbiology},
  year={2009},
  volume={47 7},
  pages={2142-8}
}
Our laboratory has developed testing methods that use real-time PCR and pyrosequencing analysis to enable the rapid identification of potential hypervirulent Clostridium difficile strains. We describe a real-time PCR assay that detects four C. difficile genes encoding toxins A (tcdA) and B (tcdB) and the binary toxin genes (cdtA and cdtB), as well as a… CONTINUE READING