Rapid method for the separation and detection of tissue short-chain coenzyme A esters by reversed-phase high-performance liquid chromatography.

@article{Demoz1995RapidMF,
  title={Rapid method for the separation and detection of tissue short-chain coenzyme A esters by reversed-phase high-performance liquid chromatography.},
  author={Abraham Demoz and Alexis Garras and Daniel K. Asiedu and Bj\orn Netteland and Rolf Kristian Berge},
  journal={Journal of chromatography. B, Biomedical applications},
  year={1995},
  volume={667 1},
  pages={148-52}
}
A simple and rapid method for the separation and identification of tissue levels of short chain coenzyme A (CoA) esters by a reversed-phase high-performance liquid chromatography with ultraviolet-visible adsorbance detection is described. Samples of liver, heart and kidney tissues were homogenised in 5% sulfosalicylic acid containing 50 microM of dithioerythritol in 1:9 w/v proportion. Following centrifugation, 20 microliters of the supernatant were directly injected onto a 3-micron ODS C18… CONTINUE READING
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