Rapid method for determining serum bactericidal activity.

@article{Provonchee1974RapidMF,
  title={Rapid method for determining serum bactericidal activity.},
  author={R B Provonchee and Stephen H. Zinner},
  journal={Applied microbiology},
  year={1974},
  volume={27 1},
  pages={185-6}
}
To screen large numbers of sera, a method was devised which utilizes the Steers-Foltz replicator which is usually used to determine minimal inhibitory concentration for antibiotics. Each of the wells (9 by 15 mm) of the replicator is filled with 0.06 ml of serum, 0.02 ml of a 10(5) suspension of organisms, and 0.02 ml of diluent (tris(hydroxymethyl)aminomethane-hydrochloride buffer, pH 8.4). The mixtures are incubated for 3 h, and samples are taken at 0, 1, 2, and 3 h by stamping duplicate… CONTINUE READING