Rapid method for automated on-line extraction and fractionation of plasma leukotrienes and 12-hydroxy-5,8,10,14-eicosatetraenoic acids by reversed-phase high-performance liquid chromatography.

@article{VanAlstyne1992RapidMF,
  title={Rapid method for automated on-line extraction and fractionation of plasma leukotrienes and 12-hydroxy-5,8,10,14-eicosatetraenoic acids by reversed-phase high-performance liquid chromatography.},
  author={Eldwin L. VanAlstyne and Stephen M. Spaethe},
  journal={Journal of chromatography},
  year={1992},
  volume={579 1},
  pages={
          37-43
        }
}
  • Eldwin L. VanAlstyne, Stephen M. Spaethe
  • Published in Journal of chromatography 1992
  • Chemistry, Medicine
  • A method is described for automated on-line extraction and fractionation of plasma leukotrienes (LTs) and (5Z,8Z,10E,14Z)-(12S)-hydroxy-5,8,10,14-eicosate traenoic acid [12(S)-HETE] by reversed-phase high-performance liquid chromatography (RP-HPLC). This method was utilized to assess the accuracy of leukotriene B4 (LTB4) and leukotriene C4 (LTC4) determinations obtained by direct immunoassay of guinea pig plasma. Plasma LTB4 levels were significantly higher (p less than 0.01) and plasma LTC4… CONTINUE READING

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