Rapid metabolic analysis of Rhodococcus opacus PD630 via parallel 13C-metabolite fingerprinting.

@article{Hollinshead2016RapidMA,
  title={Rapid metabolic analysis of Rhodococcus opacus PD630 via parallel 13C-metabolite fingerprinting.},
  author={Whitney D. Hollinshead and William R. Henson and Mary H Abernathy and Tae Seok Moon and Yinjie J. Tang},
  journal={Biotechnology and bioengineering},
  year={2016},
  volume={113 1},
  pages={91-100}
}
For rapid analysis of microbial metabolisms, (13)C-fingerprinting employs a set of tracers to generate unique labeling patterns in key amino acids that can highlight active pathways. In contrast to rigorous (13)C-metabolic flux analysis ((13)C-MFA), this method aims to provide metabolic insights without expensive flux measurements. Using (13)C-fingerprinting, we investigated the metabolic pathways in Rhodococcus opacus PD630, a promising biocatalyst for the conversion of lignocellulosic… CONTINUE READING

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