Rapid low-cost detection of Klebsiella pneumoniae carbapenemase genes by internally controlled real-time PCR.

@article{Wang2012RapidLD,
  title={Rapid low-cost detection of Klebsiella pneumoniae carbapenemase genes by internally controlled real-time PCR.},
  author={Lijun Wang and Haitong Gu and Xinxin Lu},
  journal={Journal of microbiological methods},
  year={2012},
  volume={91 3},
  pages={361-3}
}
An internally controlled real-time PCR assay based on SYBR Green was developed to screen Klebsiella pneumoniae carbapenemases (KPCs) gene containing bacteria and was validated for clinical strains or surveillance specimens. When 248 clinical samples were tested, the sensitivity and specificity of the assay were 100% and 99%, respectively. 

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