Rapid isolation and purification of photosystem I chlorophyll-binding protein from Chlamydomonas reinhardtii.

@article{Ramesh2004RapidIA,
  title={Rapid isolation and purification of photosystem I chlorophyll-binding protein from Chlamydomonas reinhardtii.},
  author={Velupillai M Ramesh and Andrew N. Webber},
  journal={Methods in molecular biology},
  year={2004},
  volume={274},
  pages={
          19-28
        }
}
The available procedures for isolation and purification of photosystem I (PSI) from Chlamydomonas reinhardtii are time consuming and usually require several hours of sucrose gradient ultracentrifugation steps. This may lead to structural and functional impairment, including release of pigments and/or dissociation of protein subunits. Moreover, it is difficult to isolate intact complexes from thylakoids containing mutated PSI that accumulate to lower levels. Hence, isolation of intact PSI core… CONTINUE READING