Rapid induction and isolation of focal adhesion complexes.

@article{Plopper1993RapidIA,
  title={Rapid induction and isolation of focal adhesion complexes.},
  author={George E. Plopper and Donald E. Ingber},
  journal={Biochemical and biophysical research communications},
  year={1993},
  volume={193 2},
  pages={571-8}
}
Focal adhesion complexes (FACs) containing integrin beta 1, talin, vinculin, talin, alpha-actinin, and paxillin formed within 15 min when round cells bound magnetic microbeads coated with integrin ligands, such as fibronectin or RGD-containing peptide, but not when coated with acetylated-low density lipoprotein. Newly formed FACs were isolated and collected for biochemical analysis using a combination of detergent extraction, sonication, dounce homogenization, and magnetic pelleting. Isolated… CONTINUE READING

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