Rapid incorporation of functional rhodopsin into nanoscale apolipoprotein bound bilayer (NABB) particles.

@article{Banerjee2008RapidIO,
  title={Rapid incorporation of functional rhodopsin into nanoscale apolipoprotein bound bilayer (NABB) particles.},
  author={Sourabh Banerjee and Thomas Huber and Thomas P. Sakmar},
  journal={Journal of molecular biology},
  year={2008},
  volume={377 4},
  pages={1067-81}
}
Human apolipoprotein A-I (apo A-I) and its engineered constructs form discoidal lipid bilayers upon interaction with lipids in vitro. We now report the cloning, expression, and purification of apo A-I derived from zebrafish (Danio rerio), which combines with phospholipids to form similar discoidal bilayers and may prove to be superior to human apo A-I constructs for rapid reconstitution of seven-transmembrane helix receptors into nanoscale apolipoprotein bound bilayers (NABBs). We characterized… CONTINUE READING
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