Rapid in situ assay for indoleacetic Acid production by bacteria immobilized on a nitrocellulose membrane.

@article{Bric1991RapidIS,
  title={Rapid in situ assay for indoleacetic Acid production by bacteria immobilized on a nitrocellulose membrane.},
  author={John M Bric and Richard Bostock and S E Silverstone},
  journal={Applied and environmental microbiology},
  year={1991},
  volume={57 2},
  pages={535-8}
}
We have developed a new assay that differentiates between indoleacetic acid (IAA)-producing and -nonproducing bacteria on a colony plate lift. Medium supplemented with 5 mM L-tryptophan is inoculated with isolates of interest, overlaid with a nitrocellulose membrane, and then incubated until bacterial colonies reach 1 to 2 mm in diameter. The membrane is removed to a filter paper saturated with Salkowski reagent and incubated until distinct red haloes form around the colonies. The colorimetric… CONTINUE READING
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