Rapid identification of methicillin-resistant Staphylococcus aureus transmission in hospitals by use of phage-derived open reading frame typing enhanced by multiplex PCR and reverse line blot assay.

@article{OSullivan2010RapidIO,
  title={Rapid identification of methicillin-resistant Staphylococcus aureus transmission in hospitals by use of phage-derived open reading frame typing enhanced by multiplex PCR and reverse line blot assay.},
  author={Matthew V. N. O'Sullivan and Fanrong Kong and Vitali Sintchenko and Gwendolyn L. Gilbert},
  journal={Journal of clinical microbiology},
  year={2010},
  volume={48 8},
  pages={
          2741-8
        }
}
The relatively high-level clonality of methicillin-resistant Staphylococcus aureus (MRSA) and its frequent high-level endemicity in nosocomial settings complicate the development of methods for rapid subtyping of MRSA strains that are capable of identifying person-to-person transmission in hospitals. Phage-derived open reading frame (PDORF) typing is an MRSA typing method targeting mobile genetic elements that was recently described and evaluated using a geographically restricted set of… CONTINUE READING
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