Rapid identification of bacterial pathogens using a PCR- and microarray-based assay

  title={Rapid identification of bacterial pathogens using a PCR- and microarray-based assay},
  author={Anna-Kaarina J{\"a}rvinen and Sanna Laakso and Pasi Piiparinen and Anne Aittakorpi and Merja Lindfors and Laura Huopaniemi and Heli Piiparinen and Minna M{\"a}ki},
  booktitle={BMC Microbiology},
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design… CONTINUE READING
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