Rapid identification of Vibrio vulnificus on nonselective media with an alkaline phosphatase-labeled oligonucleotide probe.

@article{Wright1993RapidIO,
  title={Rapid identification of Vibrio vulnificus on nonselective media with an alkaline phosphatase-labeled oligonucleotide probe.},
  author={Anita C. Wright and G A Miceli and Wendy Landry and J B Christy and W D Watkins and J. G. Morris},
  journal={Applied and environmental microbiology},
  year={1993},
  volume={59 2},
  pages={541-6}
}
An oligonucleotide DNA probe (VVAP) was constructed from a portion of the Vibrio vulnificus cytolysin gene (hylA) sequence and labeled with alkaline phosphatase covalently linked to the DNA. Control and environmental isolates probed with VVAP showed an exact correlation with results obtained with a plasmid DNA probe (derived from pCVD702) previously… CONTINUE READING