Rapid identification of Escherichia coli microcin J25 producing strains using polymerase chain reaction and colony blot hybridization.

Abstract

To screen, isolate, and characterize bacterial populations producing microcin J25, we report here two rapid, reliable, and sensitive methods, using polymerase chain reaction and colony blot hybridization with a digoxigenin-labelled probe. A sample of 26 Escherichia coli strains isolated from poultry intestinal contents was evaluated to detect the sequence… (More)

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