Rapid identification of Campylobacter jejuni from poultry carcasses and slaughtering environment samples by real-time PCR.

@article{Ivanova2014RapidIO,
  title={Rapid identification of Campylobacter jejuni from poultry carcasses and slaughtering environment samples by real-time PCR.},
  author={Mirena Ivanova and Randhir Singh and Muthu Dharmasena and Chao Gong and Albert I Krastanov and X Jiang},
  journal={Poultry science},
  year={2014},
  volume={93 6},
  pages={1587-97}
}
The objective of this study was to develop a real-time PCR assay for rapid identification of Campylobacter jejuni and to apply the method in analyzing samples from poultry processing. A C. jejuni-specific primer set targeting a portion of the C. jejuni hippuricase gene was developed. The specificity of the newly designed primer pair was verified using 5 C. jejuni strains and 20 other bacterial strains. Sensitivity was determined to be as low as 1 genome copy per reaction. A total of 73 samples… CONTINUE READING