Rapid identification of Arabidopsis insertion mutants by non-radioactive detection of T-DNA tagged genes.

@article{Ros2002RapidIO,
  title={Rapid identification of Arabidopsis insertion mutants by non-radioactive detection of T-DNA tagged genes.},
  author={Gabino R{\'i}os and Andrea Lossow and Britta Hertel and Frank Breuer and Sabine Schaefer and Melanie Broich and Tatjana Kleinow and J{\'a}n J{\'a}sik and J Winter and Alejandro Ferrando and Rosa Farr{\'a}s and Mireia Panicot and Rossana Henriques and J Mariaux and Attila Oberschall and Gergely Moln{\'a}r and Kenneth Berendzen and Vijaya Shukla and Marcel Lafos and Zsuzsanna Koncz and George P. R{\'e}dei and J. L. Schell and Csaba Koncz},
  journal={The Plant journal : for cell and molecular biology},
  year={2002},
  volume={32 2},
  pages={
          243-53
        }
}
To assist in the analysis of plant gene functions we have generated a new Arabidopsis insertion mutant collection of 90 000 lines that carry the T-DNA of Agrobacterium gene fusion vector pPCV6NFHyg. Segregation analysis indicates that the average frequency of insertion sites is 1.29 per line, predicting about 116 100 independent tagged loci in the collection. The average T-DNA copy number estimated by Southern DNA hybridization is 2.4, as over 50% of the insertion loci contain tandem T-DNA… CONTINUE READING
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