Rapid identification of Acanthamoeba from contact lens case using loop-mediated isothermal amplification method.

@article{Nonsaithong2009RapidIO,
  title={Rapid identification of Acanthamoeba from contact lens case using loop-mediated isothermal amplification method.},
  author={Darawan Nonsaithong and Rangsima Passara and Kosol Roongruangchai and Prayute Buddhirakkul and Nitaya Thammapalerd},
  journal={Experimental parasitology},
  year={2009},
  volume={121 4},
  pages={
          342-5
        }
}
A method employing loop-mediated isothermal amplification (LAMP) of 18S ribosomal RNA gene was developed to detect Acanthamoeba in contact lens cases. A prevalence of 7% (10/150) was detected, with 100% sensitivity and 100% specificity when compared with the standard culture technique. Using visual inspection of turbidity a minimum of 10pg of Acanthamoeba DNA could be detected, 10 times more sensitive than quantitative PCR employing two of the LAMP primers. The production of LAMP amplicons was… CONTINUE READING
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