Rapid identification and differentiation of yeasts by DNA and PCR fingerprinting.

@article{Lieckfeldt1993RapidIA,
  title={Rapid identification and differentiation of yeasts by DNA and PCR fingerprinting.},
  author={Elke Lieckfeldt and Wieland Meyer and Th. B{\"o}rner},
  journal={Journal of basic microbiology},
  year={1993},
  volume={33 6},
  pages={413-25}
}
We have used the techniques of DNA fingerprinting and polymerase chain reaction (PCR) with probes specific for hypervariable repetitive DNA sequences (mini- and microsatellite DNAs) to analyze 36 yeast strains belonging to 10 species and 2 genera. Using (GTG)5, (GACA)4, phage M13 DNA and the M13 sequence GAGGGTGGCGGTTCT as probes and primers, respectively, we obtained DNA polymorphisms which allowed us to discriminate 23 biotechnologically important strains of the yeast Saccaromyces cerevisiae… CONTINUE READING