Rapid identification and differentiation of Saccharomyces cerevisiae, Saccharomyces bayanus and their hybrids by multiplex PCR.

@article{Torriani2004RapidIA,
  title={Rapid identification and differentiation of Saccharomyces cerevisiae, Saccharomyces bayanus and their hybrids by multiplex PCR.},
  author={Sandra Torriani and Giacomo Zapparoli and Paola Malacrino and Giovanna Suzzi and Franco Dellaglio},
  journal={Letters in applied microbiology},
  year={2004},
  volume={38 3},
  pages={239-44}
}
AIMS To develop a multiplex PCR assay for the specific identification and differentiation of Saccharomyces cerevisiae, S. bayanus and their hybrids. METHODS AND RESULTS Two sets of primers with sequences complementary to the region YBR033w were used. A single amplicon of 1710 bp or 329 bp was obtained with species S. cerevisiae and S. bayanus, respectively, while the presence of both bands was observed in S. pastorianus because of its hybrid nature. Both amplification products were also… CONTINUE READING

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