• DOI:10.3791/50407
Corpus ID: 46122246

Rapid high throughput amylose determination in freeze dried potato tuber samples.

@article{Fajardo2013RapidHT,
  title={Rapid high throughput amylose determination in freeze dried potato tuber samples.},
  author={D. Fajardo and S. Jayanty and S. Jansky},
  journal={Journal of visualized experiments : JoVE},
  year={2013},
  volume={80}
}

D. Fajardo, S. Jayanty, S. Jansky

Published 2013

Chemistry, Medicine

Journal of visualized experiments : JoVE

This protocol describes a high through put colorimetric method that relies on the formation of a complex between iodine and chains of glucose molecules in starch. Iodine forms complexes with both amylose and long chains within amylopectin. After the addition of iodine to a starch sample, the maximum absorption of amylose and amylopectin occurs at 620 and 550 nm, respectively. The amylose/amylopectin ratio can be estimated from the ratio of the 620 and 550 nm absorbance values and comparing them… CONTINUE READING

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