Rapid, high-level expression of glycosylated rice alpha-amylase in transfected plants by an RNA viral vector.

Abstract

Tobamoviral vectors have been developed for the heterologous expression of glycoproteins in plants. The rice alpha-amylase gene (OS103) was placed under the transcriptional control of a tobamovirus subgenomic promoter in a RNA viral vector. One to two weeks after inoculation, transfected Nicotiana benthamiana plants accumulated glycosylated alpha-amylase to… (More)

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@article{Kumagai2000RapidHE, title={Rapid, high-level expression of glycosylated rice alpha-amylase in transfected plants by an RNA viral vector.}, author={Monto H. Kumagai and Jonathan Donson and Guy della-Cioppa and Ludwig Grill}, journal={Gene}, year={2000}, volume={245 1}, pages={169-74} }