Rapid generation of homologous internal standards and evaluation of data for quantitation of messenger RNA by competitive polymerase chain reaction.

@article{Anderson1997RapidGO,
  title={Rapid generation of homologous internal standards and evaluation of data for quantitation of messenger RNA by competitive polymerase chain reaction.},
  author={Karen M. Anderson and Paul Cheung and Mike D Kelly},
  journal={Journal of pharmacological and toxicological methods},
  year={1997},
  volume={38 3},
  pages={133-40}
}
Sensitive and quantitative measurement of messenger RNA (mRNA) is important for accurate assessment of gene expression. Conventional methods of mRNA measurement frequently lack the sensitivity required to detect mRNA expressed at low level, such as mRNA encoding receptors and intracellar signaling molecules. Thus, the extremely sensitive RT-PCR has become the method of choice for examination of gene expression. However, quantitation of mRNA by PCR is difficult because small variations in… CONTINUE READING

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