Rapid generation of fully human monoclonal antibodies specific to a vaccinating antigen


We describe herein a protocol for the production of antigen-specific human monoclonal antibodies (hmAbs). Antibody-secreting cells (ASCs) are isolated from whole blood collected 7 d after vaccination and sorted by flow cytometry into single cell plates. The antibody genes of the ASCs are then amplified by RT-PCR and nested PCR, cloned into expression… (More)
DOI: 10.1038/nprot.2009.3


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